Microsoft MN-820 Uživatelský manuál stáhnout pdf (Strana 5)

INTRODUCTION

The Muscle Strip Myograph System - 820MS represents the rst state-of-the-art 4-channel myograph system for muscle strips

of up to 15 mm in length. DMT developed this system for simultaneous measurement of up to 4 isolated muscle preparations,

mounted as strips, between clamp supports. Several types of mounting supports exist, depending on the user’s preferences.

Customized mounting supports can be made upon request. In addition, because of the somewhat rectangular chamber, the

myograph also can be used for the study of larger strips from other organs, including larger, longer strips of smooth muscle.

Stimulation electrodes are available (optional). The electrodes are built into the chamber cover and can be used to activate the

muscle via eld stimulation from a pulse/train stimulator, such as the DMT CS200.

The muscle bath unit is constructed of aluminum, while the centrally located rectangular chamber is stainless steel for easy

cleaning. The segment clamp supports are positioned in the chamber with one side attached to a force transducer and the other

side to a micro-manipulator. The micromanipulators permit the accurate setting of passive tension (which correlates with muscle

length) while the sensitive force transducers measure the developed isometric muscle tension during stimulation. Each unit has

individually controlled gas inow and suction. The system is automatically heated to a set temperature, up to 45° C.

Following mounting and equilibration, the optimal pre-load or passive tension for the muscle can be determined. The length of

the muscle is kept constant during the experiment; thereby any contractions measured from the isolated, mounted muscle strip

will be examined under isometric conditions. Compounds can be added directly to the chamber, and muscle tension can be

monitored to evaluate the chronotropic or inotropic effects of the compound. If desired, the buffer can be sampled for metabolic

factors during and after muscle contraction(s).

This myograph is well-suited for high-throughput analysis of muscle reactivity, especially when multiple units are arranged

conveniently side-by-side. The 820MS is ideal for drug development and testing related to striated muscle, as well as for

experiments where comparing multiple muscle samples from control and treated or gene-modied animals is required. The

multitude of data generated from the force transducers can be captured through external data acquisition via either calibrated

(ltered) or uncalibrated (unltered) analog outputs or in digital format via a serial connection.

INTRODUCTION

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Microsoft MN-820 Uživatelský manuál Strana 5